The IgM II-HA assay test kit is for the quantitative determination of IgM in serum.
The IgM is a primitive immunoglobulin that a neonate synthesizes. In adult serum, it accounts for 5 to 10 percent of the total circulating immunoglobulins. Most of the serum IgM is a pentameter of five IgM monomers, each with a molecular weight of 185,000. The monomers are attached to each other with the J chain.
IgM, an efficient complement activator, has high affinity to bacteria and erythrocytes and plays a major role in protection from Gram-negative bacteria infection. The quantification of immunoglobulin in serum is important for the diagnosis, monitoring and prognosis of chronic liver disease, infectious disease, lymphocytosis, multiple myeloma and primary and secondary immune failure.
This company’s IgM II-HA test is a specific reagent based on turbidimetric immunoassay in which a sample is mixed with the Buffer solution and Anti IgM. IgM in the sample combines specifically with anti-human IgM antibody in the Anti-IgM to yield an insoluble aggregate that causes increased turbidity.
The degree of turbidity can be measured optically and is proportional to the amount of IgM in the sample. The total precision studies give a 2.8 percent CV and 0.9 percent CV at mean concentrations of 18 and 226mg/dL respectively.
Wako Diagnostics
www.wakousa.com
Keyword: assay, IgM II-HA
